The Role of Linoleic Acid, Palmitic Acid and Leucine in Lipstatin Biosynthesis by Streptomyces Toxytricini

Department of Botany & Microbiology, Gurukul Kangri University Haridwar 249404, Uttarakhand, India ABSTRACT: Lipstatin, a new and very potent inhibitor of pancreatic lipase, was isolated from the Streptomyces toxytricini. Lipstatin contain a beta lactone structure that probably account for the irreversible lipase inhibition. As per the biosynthetic pathway the role of linoleic acid, palmitic acid and leucine in lipstatin biosynthesis by Streptomyces toxytricini was studied. Different oils were analyzed to measure the concentration of the linoleic acid and palmitic acid in the medium, and then the selected oil was used as media ingredients. While leucine was used as a media ingredient as well as feeding nutrient in the production medium. The lipstatin production depends on linoleic acid palmitic acid and leucine concentration as per biochemical pathway. But in present study it was observed that in control media the lipstatin activity was 1.108 mg/g while when we use linoleic acid and palmitic acid as media ingredients then the activity was 0.610 mg/g. Similarly in case of leucine was used as ingredient as well as feeding solution the maximum activity was observed 0.810 mg/g at 264 h. Thus, it may conclude that their is no significant impact of linoleic acid, palmitic acid and leucine as media ingredients and as a feeding solution on lipstatin production.